We are seeking an ambitious Research Associate to work on Salmonella infection and cell biology in the laboratory of Dr. Teresa Thurston at the MRC Centre for Molecular Bacteriology and Infection (MRC CMBI), based at the South Kensington campus of Imperial College London.
A key objective of the Thurston laboratory is to investigate the mechanism by which Salmonella virulence proteins (effectors) promote pathogenesis by interfering with host cell signaling and immune responses.
Our laboratory recently showed that one such protein, SteE, drives Salmonella infected macrophages into a permissive, anti-inflammatory, M2-like polarised state by an unprecedented mechanism: SteE, upon interaction with the mammalian serine/threonine kinase GSK3, causes a switch in kinase specificity so that GSK3 phosphorylates tyrosine residues on non-canonical substrates that include the transcription factor STAT3.
This research project now aims to understand the transcriptional, proteomic and metabolic cell events that result in M2-like macrophage polarisation during infection.
Duties and responsibilities
You will undertake experiments involving molecular genetics (CrispR-cas9), next generation sequencing (such as RNAseq), biochemistry and cell biology to elucidate the impact of SteE and its role in targeting host defence processes. The findings of this project will further our understanding of disease pathogenesis.
The post is funded by the Medical Research Council. You will work under the supervision of Dr Teresa Thurston and primarily be based in her group. However, the project involves a multi-disciplinary team that aims to decipher the molecular mechanism of kinase manipulation by SteE. To this end, you will work closely with our established collaborator, Dr. Katrin Rittinger and a newly appointed PDRA at The Francis Crick Institute https://www.crick.ac.uk/research/labs/katrin-rittinger.
In addition, you will be expected to undertake project management and supervise and interact with research technicians, Master’s and PhD students. It is also expected that you will draft any manuscripts arising from your findings.
You will have a PhD in biochemistry, microbiology, cell biology, immunology or a closely related discipline and be willing to work both independently and as part of a multi-disciplinary team.
You will also have a strong track record, evidenced by publication of original scientific work in refereed journals.
This is a full-time, fixed-term position for 3 years.
Candidates who have not yet been officially awarded their PhD will be appointed as Research Assistant within the salary range £36,045 – £39,183 per annum.
Should you require any further details on the role please contact: Dr. Teresa Thurston at email@example.com
For technical issues when applying online please email firstname.lastname@example.org
To apply please visit: www.imperial.ac.uk/jobs and search for MED02469